Phage survival: The biodegradability of M13 phage display library in vitro
Identifieur interne : 002336 ( Main/Exploration ); précédent : 002335; suivant : 002337Phage survival: The biodegradability of M13 phage display library in vitro
Auteurs : L'Ubomíra T Thová [Slovaquie] ; Janka Bábí Ková [Slovaquie] ; Peter Celec [Slovaquie]Source :
- Biotechnology and Applied Biochemistry [ 0885-4513 ] ; 2012-11.
English descriptors
- Teeft :
- Bacteriophage, Comenius university, Gastric, Gastric juice, Homogenate, Host strain, Human blood, Incubation, Jejunum homogenate, Microbiol, Organ homogenates, Peptide, Phage, Phage display library, Phage infectivity, Phage library, Phage particles, Phage survival, Phage therapy, Phage titer, Tissue homogenates, Uids, Urine.
Abstract
Administration of bacteriophages is used for phage therapy modulation of gut microbiome or for in vivo phage display. The aim of the study was to analyze the survival of M13 phage in different body fluids and tissues in vitro. The survival of M13 phage was measured in vitro in human blood, saliva, urine, artificial gastric juice (AGJ), and mouse homogenates of stomach, jejunum, and colon after defined time points (5, 15, or 45 Min). The plates were inspected after overnight incubation and the plaques were counted. No phage was recovered after 5 Min of incubation with AGJ. In urine, the phage survival was decreased by 44% after 5 Min of incubation (P = 0.004). In saliva, the recovered titer was decreased by 33% and 88% (P < 0.05) after 15 and 45 Min, respectively. Phage coincubation with jejunum homogenate led to significant decrease of phage titer by 72% (P < 0.01) after 15 Min and by 99% (P < 0.001) after 45 Min. Decreased survival of M13 phage depending on time of incubation was proved under several in vitro conditions, with low pH in the AGJ having the most detrimental effect on phage survival. Phage pharmacokinetics described in vitro might have applications for the use of bacteriophages in vivo.
Url:
DOI: 10.1002/bab.1050
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000F46
- to stream Istex, to step Curation: 000F46
- to stream Istex, to step Checkpoint: 000353
- to stream Main, to step Merge: 002361
- to stream Main, to step Curation: 002336
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Phage survival: The biodegradability of M13 phage display library in vitro</title><author><name sortKey="T Thova, L Ubomira" sort="T Thova, L Ubomira" uniqKey="T Thova L" first="L'Ubomíra" last="T Thová">L'Ubomíra T Thová</name></author><author><name sortKey="Babi Kova, Janka" sort="Babi Kova, Janka" uniqKey="Babi Kova J" first="Janka" last="Bábí Ková">Janka Bábí Ková</name></author><author><name sortKey="Celec, Peter" sort="Celec, Peter" uniqKey="Celec P" first="Peter" last="Celec">Peter Celec</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:2018B42BC1BD0AA41D571D7070B9EDC3E6B4FCFD</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1002/bab.1050</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-RZVCT3VB-L/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000F46</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000F46</idno><idno type="wicri:Area/Istex/Curation">000F46</idno><idno type="wicri:Area/Istex/Checkpoint">000353</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000353</idno><idno type="wicri:doubleKey">0885-4513:2012:T Thova L:phage:survival:the</idno><idno type="wicri:Area/Main/Merge">002361</idno><idno type="wicri:Area/Main/Curation">002336</idno><idno type="wicri:Area/Main/Exploration">002336</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Phage survival: The biodegradability of M13 phage display library in vitro</title><author><name sortKey="T Thova, L Ubomira" sort="T Thova, L Ubomira" uniqKey="T Thova L" first="L'Ubomíra" last="T Thová">L'Ubomíra T Thová</name><affiliation wicri:level="1"><country xml:lang="fr">Slovaquie</country><wicri:regionArea>Institute of Molecular Biomedicine, Comenius University, Bratislava</wicri:regionArea><wicri:noRegion>Bratislava</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Babi Kova, Janka" sort="Babi Kova, Janka" uniqKey="Babi Kova J" first="Janka" last="Bábí Ková">Janka Bábí Ková</name><affiliation wicri:level="1"><country xml:lang="fr">Slovaquie</country><wicri:regionArea>Institute of Molecular Biomedicine, Comenius University, Bratislava</wicri:regionArea><wicri:noRegion>Bratislava</wicri:noRegion></affiliation></author><author><name sortKey="Celec, Peter" sort="Celec, Peter" uniqKey="Celec P" first="Peter" last="Celec">Peter Celec</name><affiliation wicri:level="1"><country xml:lang="fr">Slovaquie</country><wicri:regionArea>Institute of Molecular Biomedicine, Comenius University, Bratislava</wicri:regionArea><wicri:noRegion>Bratislava</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Slovaquie</country><wicri:regionArea>Department of Molecular Biology, Comenius University, Bratislava</wicri:regionArea><wicri:noRegion>Bratislava</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Slovaquie</country><wicri:regionArea>Institute of Pathophysiology, Comenius University, Bratislava</wicri:regionArea><wicri:noRegion>Bratislava</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biotechnology and Applied Biochemistry</title><title level="j" type="alt">BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY</title><idno type="ISSN">0885-4513</idno><idno type="eISSN">1470-8744</idno><imprint><biblScope unit="vol">59</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="490">490</biblScope><biblScope unit="page" to="494">494</biblScope><biblScope unit="page-count">5</biblScope><date type="published" when="2012-11">2012-11</date></imprint><idno type="ISSN">0885-4513</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0885-4513</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Bacteriophage</term><term>Comenius university</term><term>Gastric</term><term>Gastric juice</term><term>Homogenate</term><term>Host strain</term><term>Human blood</term><term>Incubation</term><term>Jejunum homogenate</term><term>Microbiol</term><term>Organ homogenates</term><term>Peptide</term><term>Phage</term><term>Phage display library</term><term>Phage infectivity</term><term>Phage library</term><term>Phage particles</term><term>Phage survival</term><term>Phage therapy</term><term>Phage titer</term><term>Tissue homogenates</term><term>Uids</term><term>Urine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">Administration of bacteriophages is used for phage therapy modulation of gut microbiome or for in vivo phage display. The aim of the study was to analyze the survival of M13 phage in different body fluids and tissues in vitro. The survival of M13 phage was measured in vitro in human blood, saliva, urine, artificial gastric juice (AGJ), and mouse homogenates of stomach, jejunum, and colon after defined time points (5, 15, or 45 Min). The plates were inspected after overnight incubation and the plaques were counted. No phage was recovered after 5 Min of incubation with AGJ. In urine, the phage survival was decreased by 44% after 5 Min of incubation (P = 0.004). In saliva, the recovered titer was decreased by 33% and 88% (P < 0.05) after 15 and 45 Min, respectively. Phage coincubation with jejunum homogenate led to significant decrease of phage titer by 72% (P < 0.01) after 15 Min and by 99% (P < 0.001) after 45 Min. Decreased survival of M13 phage depending on time of incubation was proved under several in vitro conditions, with low pH in the AGJ having the most detrimental effect on phage survival. Phage pharmacokinetics described in vitro might have applications for the use of bacteriophages in vivo.</div></front></TEI><affiliations><list><country><li>Slovaquie</li></country></list><tree><country name="Slovaquie"><noRegion><name sortKey="T Thova, L Ubomira" sort="T Thova, L Ubomira" uniqKey="T Thova L" first="L'Ubomíra" last="T Thová">L'Ubomíra T Thová</name></noRegion><name sortKey="Babi Kova, Janka" sort="Babi Kova, Janka" uniqKey="Babi Kova J" first="Janka" last="Bábí Ková">Janka Bábí Ková</name><name sortKey="Celec, Peter" sort="Celec, Peter" uniqKey="Celec P" first="Peter" last="Celec">Peter Celec</name><name sortKey="Celec, Peter" sort="Celec, Peter" uniqKey="Celec P" first="Peter" last="Celec">Peter Celec</name><name sortKey="Celec, Peter" sort="Celec, Peter" uniqKey="Celec P" first="Peter" last="Celec">Peter Celec</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002336 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002336 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:2018B42BC1BD0AA41D571D7070B9EDC3E6B4FCFD
|texte= Phage survival: The biodegradability of M13 phage display library in vitro
}}
| This area was generated with Dilib version V0.6.33. |  |